Multi-drug resistance in early and late onset neonatal sepsis in a tertiary hospital in Nigeria

  • Lateefat O. Sa’adu | lateemayaki88@gmail.com Department of Medical Microbiology and Parasitology, University of Ilorin Teaching Hospital; Assisted Reproductive Technology Unit, University of Ilorin Teaching Hospital, Nigeria.
  • Tope O. Obasa Department of Pediatric and Child Health, Faculty of Clinical Sciences, College of Health Sciences, University of Ilorin, Kwara State, Nigeria.
  • Aishat O. Saka Department of Pediatric and Child Health, Faculty of Clinical Sciences, College of Health Sciences, University of Ilorin, Kwara State, Nigeria.
  • Mohammed J. Saka Department of Epidemiology and Community Health, Faculty of Clinical Sciences, College of Health Sciences, University of Ilorin, Kwara State, Nigeria.
  • Charles Nwabuisi Department of Medical Microbiology and Parasitology, University of Ilorin Teaching Hospital; Department of Medical Microbiology and Parasitology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin, Kwara State, Nigeria.

Abstract

Neonatal sepsis is a clinical syndrome characterized by systemic signs of infection, and accompanied by bacteremia in the first month of life and is responsible for 30-50% of total neonatal deaths, each year in developing countries. This study investigated multi-drug resistant organisms associated with early and late onset neonatal sepsis in the University of Ilorin Teaching Hospital (UITH). It was a descriptive cross-sectional study. One hundred and sixty-two blood samples from neonates admitted into the neonatal intensive care unit of UITH with clinical diagnosis of sepsis were obtained. One milliliter of blood was taken per neonate and cultured aerobically in brain heart infusion broth and sub cultured onto blood and MacConkey agar plates. Identification of the isolates was carried out by colonial morphology, Gram stain microscopy and several biochemical tests. Antibiotic susceptibility test was done using the modified Kirby-Bauer method, screening for methicillin resistance Staphylococcus aureus (MRSA) and extended spectrum beta lactamase (ESBL) was done by the cefoxiin- based methods and double disc synergy test respectively. Data analysis was carried out using Microsoft excel version 2007 and Epi-info version 2012. Sepsis was confirmed bacteriologically in 22.2% of the samples. The prevalence of multidrug resistant isolate was 29.0%. The prevalence of MRSA was found to be 37% while that of ESBL producing Enterobacteria was 44.4% with ESBL producing Klebsiella pneumoniae and Escherichia coli prevalence of 50% and 25% respectively. This study shows a high prevalence of Methicillin Resistant Staphylococcus Aureus and Extended Spectrum Beta Lactamase producing Klebsiella pneumoniae and Escherichia coli causing neonatal sepsis in UITH Ilorin.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.
Published
2020-01-20
Info
Issue
Section
Articles
Keywords:
Neonatal Sepsis, Early Onset sepsis, Late onset sepsis, Methicillin resistance Staphylococcus aureus, Extended spectrum beta lactamase
Statistics
  • Abstract views: 298

  • PDF: 110
How to Cite
Sa’adu, Lateefat O., Tope O. Obasa, Aishat O. Saka, Mohammed J. Saka, and Charles Nwabuisi. 2020. “Multi-Drug Resistance in Early and Late Onset Neonatal Sepsis in a Tertiary Hospital in Nigeria”. Annals of African Medical Research 2 (2). https://doi.org/10.4081/aamr.2019.69.